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Read e-book online Advances in Microbial Physiology, Vol. 20 PDF

By A. H. & Morris, J. Gareth [eds.] Rose

This quantity in a research-level sequence covers diversified elements of microbial body structure and biochemistry together with inositol metabolisms in yeasts, bacterial adhesion, natural acids, the bacterial flagellum and the mechanical behaviour of bacterial mobile partitions. it's meant to be of use to microbiologists, biochemists and biotechnologists. different comparable works during this sequence are volumes 29, 30 and 31.

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I n cells sequentially labelled with [32P] orthophosphate and r3H] uracil, 23s fragmentation is always most extensive in the “older” (first-labelled) rRNA. Pulse-chase kinetics show an exponential decay (tf approximately 5 h) in that fraction of total 23Srelated material which migrates as intact 23s rRNA on polyacrylamide gels, and a concomitant equimolar rise in the fractions represented by each of the fragments. It seems most likely that chain-scission is a random in uivo event; each intact 50s subunit in an exponentially-growing culture having an equal, age-independent, probability of suffering endonucleolytic attack.

2. Engmology I can find but one report describing DNA-dependent DNA polymerase activity in cvanohacteria (Schonherr and Keir, 1972). Anabaena variabilis and A. nidufans were shown to contain enzymes which required, for optimal activity, low magnesium, high pH and all four 28 W. FORD DOOLITTLE deoxyribonucleoside triphosphates. Denatured DNA proved a better template than did native DNA, and deoxyribonuclease activities in the preparations were low. 3. DNA Replication and the Cell Cycle There is a considerable literature bearing more-or-less directly on the regulation of cyanobacterial DNA replication.

5 x lo9 dalton genome occurred during the evolution of modern bacteria from a primitive mycoplasm-like ancestor, while the distribution of functionally related genetic markers on the linkage maps of Streptomyces coelicolor and E. coli are consistent with the notion that these arose by duplication or quadruplication (Hopwood, 1967; Zipkas and Riley, 1975). The suggestion that structural or functional complexity requires a large genome bears thought. , 1977). In cyanobacteria, filamentous forms can arise quite easily by mutation from unicellular species (Ingram and Van Baalen, 1970; Kunisawa and Cohen-Bazire, 1970), and the phylogenetic distance between even highly-differentiated, heterocyst-bearing filamentous species and simple unicellular forms may not be very great (Bonen and Doolittle, 1978).

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